Electrophoretic studies of red cell extracts of stored blood.

It has been shown that red cells of blood collected in acid-citrate-dextrose (ACD) undergo changes in their dimensions, osmotic fragility, permeability and metabolism during storage at 4° C (1). Information concerning the effect of storage on the physical and chemical states of soluble red cell proteins, particularly hemoglobin, is not available. Such data may be of interest in view of the marked changes observed in the mobility and concentration of several proteins during the storage of plasma (2). This paper presents evidence from electrophoretic analyses that the concentration of components of extracts of red cells is changed during storage of blood.